|
NextSeq2000 files
|
|
26
|
602
|
September 24, 2023
|
|
Issue with pre.cluster
|
|
10
|
416
|
October 30, 2023
|
|
Co-ordinates for aligning v5-v7 region
|
|
13
|
331
|
September 1, 2023
|
|
Generating ASVs that all have a size of one
|
|
12
|
308
|
October 10, 2023
|
|
Errors in cluster.split
|
|
11
|
271
|
December 28, 2023
|
|
Help with contigs
|
|
10
|
284
|
August 21, 2023
|
|
Recurring "Your count table contains more than 1 sequence named..." yet doesn't
|
|
12
|
254
|
July 16, 2023
|
|
Get.oturep using abundance method
|
|
11
|
250
|
October 27, 2023
|
|
Running mothur in hpc with conda
|
|
10
|
257
|
October 10, 2023
|
|
Segmentation fault in pcr.seqs
|
|
9
|
232
|
September 23, 2023
|
|
Customize Silva reference for V4 region
|
|
6
|
368
|
August 13, 2023
|
|
Dist.seqs, cluster.split and split.abund taking long time
|
|
3
|
671
|
May 12, 2023
|
|
Several sequences missing primers and many ambiguous base calls
|
|
7
|
282
|
November 24, 2023
|
|
Classify.seqs error ugreen db for 23S
|
|
5
|
310
|
July 23, 2023
|
|
[ERROR} Your count table contains more than 1 sequence named
|
|
5
|
346
|
September 21, 2023
|
|
Customize coordinates for v4 region
|
|
6
|
263
|
June 2, 2023
|
|
Errors following align.seqs
|
|
8
|
208
|
January 26, 2024
|
|
Alignment issues
|
|
4
|
302
|
September 5, 2023
|
|
Problem with make.file
|
|
5
|
272
|
August 7, 2023
|
|
File mismatch detected in summary.seqs
|
|
6
|
247
|
June 4, 2023
|
|
Stuck at clustering, its running for more than a week
|
|
7
|
212
|
January 20, 2024
|
|
Installing mothur error
|
|
7
|
195
|
December 5, 2023
|
|
Overlapping V3-V4 using v2 2x250 bp
|
|
3
|
304
|
October 18, 2023
|
|
MiDAS database for Mothur
|
|
5
|
230
|
July 22, 2023
|
|
Discrepancy between number of reads and group counts from make.contigs
|
|
6
|
198
|
July 6, 2023
|
|
OTURare vs taxonomy
|
|
5
|
244
|
June 24, 2023
|
|
Demultiplex dual indexed Illumina files
|
|
7
|
175
|
February 22, 2024
|
|
Segfault running screen.seqs in WDL workflow
|
|
3
|
281
|
June 17, 2023
|
|
Salmonella, again
|
|
6
|
197
|
June 16, 2023
|
|
Help in pre.cluster
|
|
3
|
267
|
August 18, 2023
|
|
Dist.seqs: your sequences are not the same length, aborting
|
|
2
|
306
|
June 29, 2023
|
|
LefSe error: requesting groups not present in files, aborting
|
|
4
|
225
|
June 4, 2023
|
|
Unique.seqs ERROR after make.contigs
|
|
4
|
217
|
August 31, 2023
|
|
Problem with filter.seqs - Length of filtered alignment: 0
|
|
4
|
305
|
June 22, 2023
|
|
ASV and OTU same number of sequences
|
|
2
|
308
|
October 12, 2023
|
|
Issue with venn command
|
|
3
|
232
|
November 2, 2023
|
|
What is the minimum required overlap to form a contig?
|
|
4
|
208
|
September 7, 2023
|
|
Error with remove.otus
|
|
4
|
213
|
November 12, 2023
|
|
Filter.seqs error: Sequences are not all the same length
|
|
2
|
220
|
January 9, 2024
|
|
Missing denovo.vsearch.fasta file
|
|
4
|
209
|
June 1, 2023
|
|
Pre.cluster in unaligned, or cluster split?
|
|
4
|
187
|
December 10, 2023
|
|
Looking for the coordinates of ITS2 region based sequencing
|
|
2
|
283
|
June 24, 2023
|
|
Using pcr.seqs without a forward primer in the oligo file results in first four bases being removed
|
|
2
|
253
|
October 23, 2023
|
|
Hi, I'm working 16s rRNA analysis. Recently I got my Illumina sequences for my sample. I have my sequence with 001_R1 AND 001_R2 with 155 dual sample sequence. Do I need to rename the fastq files or I should make some oligo for making stability files
|
|
5
|
160
|
January 12, 2024
|
|
V4 region - Archaea and Bacteria at genus level
|
|
3
|
226
|
September 14, 2023
|
|
Chimera vsearch error
|
|
8
|
120
|
April 24, 2024
|
|
Generating cons.tax.summary
|
|
4
|
188
|
August 21, 2023
|
|
Count.groups discrepancy?
|
|
3
|
210
|
December 2, 2023
|
|
Using a custom taxonomic database
|
|
3
|
223
|
September 5, 2023
|
|
How to show number of reads in each sample?
|
|
3
|
217
|
June 26, 2023
|