Problem with make.file

I get the following error when trying to use the make.file function on the provided MiSeq_SOP data:

[ERROR]: basic_string::replace: __pos (which is 4294967295) > this->size() (which is 74)RAM used: 13.3957Gigabytes . Total Ram: 15.7289Gigabytes.

The command i use is:
mothur > make.file(inputdir=mothurhome, type=fastq, prefix=stability)

the files are located in the same folder as the mothur.exe (using windows 10). I don’t seem to get an output file…?

Does anyone have any suggestion to what is wrong and what I can do? I am completely new to mothur so appreciate any help!

Hmmm, something weird is definitely going on. Could you post a screenshot of the mothurhome directory?


Here is the mothurhome directory. And if it is of any help, I ran the exact same thing on a Linux (ubuntu 22.04) machine without any problems


Could you try again after removing the __MACOSX and .DS_Store files? Also, instead of doing mothurhome could you use ./ instead?

That fixed the problem! Thank you!

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