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Welcome to the mothur forum
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1
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5644
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January 29, 2026
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MiSeq i100 "SOP"
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8
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88
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February 11, 2026
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Screen.seqs generating weird result
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1
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8
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February 9, 2026
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Remove dominant OTU
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2
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18
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January 21, 2026
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Classify.seqs with Pacbio/long reads not classifying any taxa
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2
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37
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December 26, 2025
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Cluster.split in mothur
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2
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24
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December 25, 2025
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16S sequences longer than 251 bp
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6
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63
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December 22, 2025
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Sub.sample in mothur
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4
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41
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December 22, 2025
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Analyzing two different runs separately
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2
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35
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December 21, 2025
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Get sequences from OTUs
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2
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31
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December 15, 2025
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Trimming ambiguous ends16S
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4
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67
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December 11, 2025
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Combining sampling times
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2
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70
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November 20, 2025
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Predictive Metagenomics: Rarefied or Raw
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5
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86
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November 11, 2025
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What's the best experimental design for targeted metagenomics?
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2
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56
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October 30, 2025
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Summary.seqs error
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1
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34
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November 8, 2025
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Minimal R relative abundance question
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8
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93
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November 6, 2025
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Class is missing from the lefse output
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5
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53
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November 2, 2025
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Mixed 16S/ITS kit but reads look overwhelmingly V3–V4 — how to verify and subset per region?
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1
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23
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October 16, 2025
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Rarefaction in mothursop
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2
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61
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October 24, 2025
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Appropriate length filtering strategy for 16S V4–V5 (515F–907R) amplicons: percentile vs fixed cutoffs?
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4
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83
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October 19, 2025
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Error during pcr.seqs step: Error in reading your fastafile, at position -1. Blank name
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6
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94
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October 11, 2025
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You did not provide a class
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10
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115
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October 10, 2025
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UD Index's and amplicon length
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6
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73
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October 2, 2025
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Sub.sample output use
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2
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56
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September 30, 2025
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Compare Samples With Sequences of Different Lengths?
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4
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80
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September 4, 2025
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AMOVA Tutorial Sample design File Missing Headers
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0
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30
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August 25, 2025
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Issue with bacterial classification using Mothur v1.48.3 and SILVA v138.2
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3
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45
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August 29, 2025
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Align.seq Process Killed
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3
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48
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August 29, 2025
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Pcr.seqs command error
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1
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26
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August 1, 2025
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Classify.seqs() marking most sequences as unclassified
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3
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69
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August 7, 2025
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