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Welcome to the mothur forum
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0
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5660
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October 10, 2018
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Make.contigs doesn’t create stability.contigs.count_table
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7
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16
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March 19, 2026
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Chimera.vsearch does not update count table
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3
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19
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March 8, 2026
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Trim.seqs removes group membership
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4
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16
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March 15, 2026
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Screen.seqs generating weird result
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8
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38
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March 8, 2026
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MiSeq i100 "SOP"
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8
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116
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February 11, 2026
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Remove dominant OTU
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2
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25
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January 21, 2026
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Classify.seqs with Pacbio/long reads not classifying any taxa
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2
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61
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December 26, 2025
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Cluster.split in mothur
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2
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38
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December 25, 2025
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16S sequences longer than 251 bp
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6
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92
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December 22, 2025
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Sub.sample in mothur
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4
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58
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December 22, 2025
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Analyzing two different runs separately
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2
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48
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December 21, 2025
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Get sequences from OTUs
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2
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43
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December 15, 2025
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Trimming ambiguous ends16S
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4
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82
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December 11, 2025
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Combining sampling times
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2
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81
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November 20, 2025
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Predictive Metagenomics: Rarefied or Raw
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5
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104
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November 11, 2025
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What's the best experimental design for targeted metagenomics?
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2
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66
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October 30, 2025
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Summary.seqs error
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1
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42
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November 8, 2025
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Minimal R relative abundance question
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8
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107
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November 6, 2025
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Class is missing from the lefse output
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5
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70
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November 2, 2025
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Mixed 16S/ITS kit but reads look overwhelmingly V3–V4 — how to verify and subset per region?
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1
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38
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October 16, 2025
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Rarefaction in mothursop
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2
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76
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October 24, 2025
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Appropriate length filtering strategy for 16S V4–V5 (515F–907R) amplicons: percentile vs fixed cutoffs?
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4
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105
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October 19, 2025
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Error during pcr.seqs step: Error in reading your fastafile, at position -1. Blank name
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6
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104
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October 11, 2025
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You did not provide a class
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10
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129
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October 10, 2025
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UD Index's and amplicon length
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6
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91
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October 2, 2025
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Sub.sample output use
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2
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76
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September 30, 2025
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Compare Samples With Sequences of Different Lengths?
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4
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90
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September 4, 2025
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AMOVA Tutorial Sample design File Missing Headers
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0
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38
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August 25, 2025
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Issue with bacterial classification using Mothur v1.48.3 and SILVA v138.2
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3
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60
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August 29, 2025
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