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Welcome to the mothur forum
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0
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5688
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October 10, 2018
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Demultiplexing Minion data
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4
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28
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August 8, 2026
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Gaps "misplaced" with align seqs
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8
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77
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July 17, 2026
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AWS EC2 Community mothur AMI - still an option?
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3
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42
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July 1, 2026
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Customizing latest SILVA for V4
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3
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58
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May 21, 2026
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Agglomerating OTUs by taxonomic rank
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2
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43
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May 4, 2026
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Make.contigs doesn’t create stability.contigs.count_table
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7
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53
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March 19, 2026
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Chimera.vsearch does not update count table
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3
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49
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March 8, 2026
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Trim.seqs removes group membership
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3
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47
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March 5, 2026
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Screen.seqs generating weird result
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7
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91
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February 26, 2026
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MiSeq i100 "SOP"
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8
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263
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February 11, 2026
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Remove dominant OTU
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2
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48
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January 21, 2026
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Classify.seqs with Pacbio/long reads not classifying any taxa
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1
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91
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December 16, 2025
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Cluster.split in mothur
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1
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52
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December 15, 2025
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16S sequences longer than 251 bp
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5
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125
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December 12, 2025
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Sub.sample in mothur
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3
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78
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December 12, 2025
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Analyzing two different runs separately
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1
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62
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December 11, 2025
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Get sequences from OTUs
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1
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51
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December 5, 2025
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Trimming ambiguous ends16S
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3
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103
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December 1, 2025
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Combining sampling times
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1
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88
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November 6, 2025
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Predictive Metagenomics: Rarefied or Raw
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4
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124
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November 1, 2025
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What's the best experimental design for targeted metagenomics?
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2
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84
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October 30, 2025
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Summary.seqs error
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0
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54
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October 29, 2025
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Minimal R relative abundance question
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7
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141
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October 27, 2025
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Class is missing from the lefse output
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4
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98
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October 23, 2025
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Mixed 16S/ITS kit but reads look overwhelmingly V3–V4 — how to verify and subset per region?
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1
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48
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October 16, 2025
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Rarefaction in mothursop
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1
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97
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October 14, 2025
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Appropriate length filtering strategy for 16S V4–V5 (515F–907R) amplicons: percentile vs fixed cutoffs?
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3
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166
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October 9, 2025
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Error during pcr.seqs step: Error in reading your fastafile, at position -1. Blank name
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5
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124
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October 1, 2025
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You did not provide a class
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9
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165
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September 30, 2025
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