Hi. Last week I downloaded and used the RDP v19 reference files (RDP version), and had no troubles. Today, after doing a classify.seqs after downloading it again, it showed the following error:
mothur > classify.seqs(fasta=seqs.trim.contigs.fasta, reference=trainset19_072023.rdp.fasta, taxonomy=trainset19_072023.rdp.tax, cutoff=80)
Using 32 processors.
Generating search database… DONE.
It took 12 seconds generate search database.
Reading in the trainset19_072023.rdp.tax taxonomy… [ERROR]: AJ000684 is missing the final ‘;’, ignoring.
[ERROR]: EF599163 is missing the final ‘;’, ignoring.
[ERROR]: AY859683 is missing the final ‘;’, ignoring.
[ERROR]: AY883036 is missing the final ‘;’, ignoring.
[ERROR]: DQ656489 is missing the final ‘;’, ignoring.
[ERROR]: D85479 is missing the final ‘;’, ignoring.
[ERROR]: DQ915443 is missing the final ‘;’, ignoring.
[ERROR]: EF626689 is missing the final ‘;’, ignoring.
[ERROR]: AB081760 is missing the final ‘;’, ignoring.
[ERROR]: AB006176 is missing the final ‘;’, ignoring.
[ERROR]: AB273741 is missing the final ‘;’, ignoring.
DONE.
‘AJ000684’ is in your template file and is not in your taxonomy file. Please correct.
‘EF599163’ is in your template file and is not in your taxonomy file. Please correct.
‘AY859683’ is in your template file and is not in your taxonomy file. Please correct.
‘AY883036’ is in your template file and is not in your taxonomy file. Please correct.
‘DQ656489’ is in your template file and is not in your taxonomy file. Please correct.
After looking into the new .fasta and .tax files, I found that the .tax file is missing the ; after the taxonomies, which is present in the previous versions.
Best regards,
Douglas