Unable to open silva.seed_v138.1

Hello, from jenam who is a fresh man for bioinformatics :slight_smile:

I have some troubles with my work

Iā€™d like to do sequence alignment for 16s rRNA gene sequences

I guess I have to set my directory for all analysis files, right?

Want to know what I have to do firstly :slight_smile:

Plz help :slight_smile:

Hi,

Iā€™d encourage you to put all of your data files, reference files, and mothur program in a single directory - there should be only files in that directory, no directories. Then try double clicking on the mothur executable and run the commands.

Pat

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