missing silva.nr_v123.8mer file

mothur bugs
1

Hi there,
I’m currently using the 454 SOP to work through my data and on the align.seqs command, I’m getting an error with my current install of mothur v.1.36.0:

mothur > align.seqs(fasta=/users/jw698/tamar/data/Plate2_Lane1.shhh.trim.unique.fasta, reference=silva.nr_v123.align, processors=2)

Using 2 processors.
Unable to open silva.nr_v123.align. Trying default /cm/shared/apps/mothur/1.36.0/blast/bin/silva.nr_v123.align

Reading in the /cm/shared/apps/mothur/1.36.0/blast/bin/silva.nr_v123.align template sequences... DONE.
It took 276 to read  172418 sequences.
[ERROR]: Could not open /cm/shared/apps/mothur/1.36.0/blast/bin/silva.nr_v123.8mer

I have copied all the Silva reference files from here to the mothur/1.36.0/blast/bin/ directory, but I can’t find any *.8mer files that I might be missing.

Any ideas?

Thanks,
Ben

2

I’m not sure where you’re trying to run this from, but could you try this?

mothur > align.seqs(fasta=/users/jw698/tamar/data/Plate2_Lane1.shhh.trim.unique.fasta, reference=/cm/shared/apps/mothur/1.36.0/blast/bin/silva.nr_v123.align, processors=2)

Pat