Hi,
My Mothur is unable to read the silva reference files in align.seqs, please help…
using Mothur 1.34.4 in Amazon EC2 linux server, I get the following results:
mothur> align.seqs(fasta=stability.trim.contigs.good.unique.fasta, reference=silva.seed_v119.align, flip=T, processors=4)
using 4 processors.
Reading in the silva.seed_v119.align template sequences… [ERROR]: template is not aligned, aborting.
DONE.
It took 14 to read 10281 sequences.
mothur> align.seqs(fasta=stability.trim.contigs.good.unique.fasta, reference=silva.nr_v119.align, flip=T, processors=4)
using 4 processors.
Reading in the silva.nr_v119.align template sequences… Killed
I was thinking as a last resort to try using an older version of Mothur, but these are not compiled and I don’t know enough about compiling.
Thanks for your help,
P.