Dist.seqs: your sequences are not the same length, aborting

Commands in mothur
1

I ran into an error message “your sequences are not the same length, aborting” when I tried running the dist.seqs command.

My sequences initially had different lengths, so I thought I could fix it by

  • use filter.seq (same directory)

  • adjusting the minlength parameter (screen.length) (rerun from scratch)

However, that didn’t solve the problem
I’m not sure if I made the right adjustment and any additional advice you may have. Thank you.

2

Hi - can you post your entire pipeline? You would normally make everythign the same length by aligning them using align.seqs

Pat

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