Generating cons.tax.summary

Commands in mothur
1

Hi all,
I don’t know if it was written here, although I didn’t find, but
how can I generate the file cons.tax.summary after subsampling to the least number of sequences?
I know using shared and taxonomy I can make the correspondence of OTU/ASV, however I also like to use the cons.tax.summary as input for relative abundance chart.

Thanks

2

You don’t need to update the cons.taxonomy file after subsampling. The “name” of the OTU shouldn’t change.

3

but in case i would like to set the samples by tax level (eg. family or genus) using the same number of sequences? i cannot generate a tax.summary after subsampling? (sorry but in the title of this topic I’ve mentioned cons.tax file)

thanks

4

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5

You can generate a shared file and get the consensus taxonomy for each phylotypes like we do in the phylotype section

Thanks,
Pat