|
Tree file for ASV
|
|
1
|
203
|
December 5, 2023
|
|
Using ASV or OTU in 16s analysis
|
|
1
|
379
|
December 5, 2023
|
|
File mismatch detected after align.seqs or screen.seqs
|
|
1
|
347
|
December 5, 2023
|
|
Installing mothur error
|
|
7
|
287
|
December 5, 2023
|
|
Beginner questions: Working step for organism identification
|
|
1
|
241
|
November 30, 2023
|
|
Pre.cluster in unaligned, or cluster split?
|
|
3
|
254
|
November 30, 2023
|
|
Pcr.seqs does not remove logical amount of sequences with different values of pdiffs/rdiffs
|
|
14
|
831
|
November 28, 2023
|
|
Count.groups discrepancy?
|
|
2
|
267
|
November 22, 2023
|
|
Several sequences missing primers and many ambiguous base calls
|
|
6
|
489
|
November 14, 2023
|
|
Lefse: no class column with more than 2 classes
|
|
0
|
203
|
November 14, 2023
|
|
The best region for 16S sequencing
|
|
1
|
268
|
November 2, 2023
|
|
Error with remove.otus
|
|
3
|
294
|
November 2, 2023
|
|
Issue with venn command
|
|
3
|
296
|
November 2, 2023
|
|
Phylotypes classification at different taxonomic levels
|
|
1
|
226
|
October 30, 2023
|
|
Preparing for analysis
|
|
1
|
243
|
October 30, 2023
|
|
Get.oturep using abundance method
|
|
11
|
352
|
October 27, 2023
|
|
Using pcr.seqs without a forward primer in the oligo file results in first four bases being removed
|
|
2
|
334
|
October 23, 2023
|
|
Issue with pre.cluster
|
|
9
|
638
|
October 20, 2023
|
|
Alignment command
|
|
2
|
268
|
October 18, 2023
|
|
Overlapping V3-V4 using v2 2x250 bp
|
|
3
|
496
|
October 18, 2023
|
|
Unique names issue
|
|
2
|
256
|
October 18, 2023
|
|
Make.contigs is not creating Group file
|
|
1
|
360
|
October 18, 2023
|
|
Running mothur in hpc with conda
|
|
10
|
578
|
October 10, 2023
|
|
Generating ASVs that all have a size of one
|
|
12
|
513
|
October 10, 2023
|
|
Difference between Phylotype and OTUs
|
|
1
|
386
|
October 2, 2023
|
|
ASV and OTU same number of sequences
|
|
1
|
437
|
October 2, 2023
|
|
Database contains Oomycete
|
|
1
|
299
|
September 18, 2023
|
|
V4 region - Archaea and Bacteria at genus level
|
|
3
|
335
|
September 14, 2023
|
|
Keeping OTUs/ASVs with filter.shared
|
|
1
|
272
|
September 14, 2023
|
|
NextSeq2000 files
|
|
25
|
846
|
September 14, 2023
|