I am analyzing 16S sequencing data from region v4 bacteria, so I am following all the steps available in the tutorial on the mothur website. However, I have to skip the part about accessing the error rate because I didn’t evaluate it, after that I can’t run anything else, is anyone in the same situation?
Hi Amanda - you should be fine to proceed. You’ll need to update the filenames found on the wiki to match what you have. You’ll likely want to drop the last
pick from the filenames
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