I’m fairly new to microbiology and analyzing microbial community samples. I’ve been working through the example analyses for a couple of days now, and I can manage to process my pyrosequencing data.
However, I need to compare it to sequences obtained through cultivation.
How should I do this using mothur? How can I analyze the data both within and between samples AND techniques? Should I merge the data, and then just go through with ‘standard’ analysis?
I’ve been suggested to map the pyrosequences on the Sanger sequences, and get a table with presence/absence for both techniques per sequence, and then use this for ordination. Is this possible with mothur?
Something like this:
sequence pyrosequence cultivation 1 present present 2 absent present etc.
Sorry for these very basic questions. If you know any good step-by-stap tutorials on how to start with this kind of analysis, please let me know. :oops:
Thanks in advance!
ps: I don’t know if this is the right section?