Hello everyone, I have been trying to use Mothur to process 16S rRNA sequence data. Whenever I got to the line of codes below, the run is aborted. Have you encountered this before? Your assistance is highly appreciated. Jean-Rene
cluster.split(fasta=current, count=current, taxonomy=current, splitmethod=classify, taxlevel=4, cutoff=0.15)
Hi there,
I suspect you are using up all of the RAM on your computer. Can you tell me what region you are sequencing? You might try to drop the cutoff to 0.03, which should help a bit.
Pat