"cluster.split"

1

mothur > cluster.split(fasta=final.fasta, count=final.count_table, taxonomy=final.taxonomy)

Using 128 processors.
Splitting the file…
/*****************************************/
Selecting sequences for group Gammaproteobacteria (1 of 25)
Number of unique sequences: 894086
Selected 2453200 sequences from final.count_table.
Calculating distances for group Gammaproteobacteria (1 of 25):
Sequence Time Num_Dists_Below_Cutoff
0 1 0
100 2 134
200 4 590
300 6 1549
400 10 3060
It is taking more time and not finishing.
My run has 120 samples with v3&v4 amplified genes of 16S rRNA with paired end sequences (2250).
Thank You,

2

With the region you are using I think you are running into this problem…

You might just sit tight and see if the data processes if you wait long enough
Pat

3

Thank You Pat for your reply.
In this case, If I wait long, will it process my data correctly?

Thank You

4

Yep, these things take time