Sir, I have started this clustering step but my cursor was not blinking so i am doubtful that is it working or not. 3hrs completed since i have ran it.
Actually earlier when i did clustering step my cursor always blinks until its finished. So please look into this.
@pschloss @westcott sir
Thank You
It’s probably still thinking. I’d suggest using cluster.split instead if it’s taking more than 20 hours
Pat
Sir, is cluster.split will provide same output file that cluster will going to provide?
so for all taxonomy levels i will ran cluster.split command 6 times?
This likely means that you ran out of RAM. What region are you sequencing and with what technology/chemistry? I’d encourage you to read:
Pat
Please look at the MiSeq SOP. You will only run it for one taxonomic level and with one distance threshold.
Pat
Sir, we have sequenced V3-V4 region. What more things i need to tell about our data so that i can get more help from you.
Thank you
The problem is that you have sequenced a region where you do not have fully overlapping sequence reads. This results in an inflated error rate and will get a large number of spurious OTUs. You need to read this…
Pat
Ok sir i will go through this link.
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