The best region for 16S sequencing

Dear all. I’ve read a recent paper about v1-v2 as the best region for 16S analysis. However I also noticed here about v4 being the best one… what is your opinion about that in a recent perspective?!

Thank you in advance

There are many factors that figure in to the “best” region. These would include the length, quality of primers, taxonomic signal provided by the region. The V4 hits all of these for me. The most important to me is length because I want to have two reads that fully overlap with each other to get full denoising of any sequencing errors.


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