|
Using pcr.seqs without a forward primer in the oligo file results in first four bases being removed
|
|
2
|
240
|
October 23, 2023
|
|
Alignment command
|
|
2
|
191
|
October 18, 2023
|
|
Overlapping V3-V4 using v2 2x250 bp
|
|
3
|
290
|
October 18, 2023
|
|
Unique names issue
|
|
2
|
186
|
October 18, 2023
|
|
Running mothur in hpc with conda
|
|
10
|
240
|
October 10, 2023
|
|
Generating ASVs that all have a size of one
|
|
12
|
301
|
October 10, 2023
|
|
Database contains Oomycete
|
|
1
|
221
|
September 18, 2023
|
|
V4 region - Archaea and Bacteria at genus level
|
|
3
|
214
|
September 14, 2023
|
|
Alignment issues
|
|
4
|
293
|
September 5, 2023
|
|
Using a custom taxonomic database
|
|
3
|
213
|
September 5, 2023
|
|
Co-ordinates for aligning v5-v7 region
|
|
13
|
312
|
September 1, 2023
|
|
Lefse [ERROR]: requesting groups not present in files, aborting. Segmentation fault: 11
|
|
9
|
537
|
August 27, 2023
|
|
Phylogenetic Tree File
|
|
1
|
203
|
July 25, 2023
|
|
Blank file after screen
|
|
1
|
173
|
July 25, 2023
|
|
Help formatting fasta for mock community
|
|
1
|
181
|
July 25, 2023
|
|
how tp get asv file
|
|
1
|
197
|
July 11, 2023
|
|
Discrepancy between number of reads and group counts from make.contigs
|
|
6
|
188
|
July 6, 2023
|
|
Help! Archaea microbial community
|
|
1
|
186
|
June 27, 2023
|
|
Connecting to AWS instance via Putty or SSH
|
|
0
|
277
|
June 22, 2023
|
|
Archaea community
|
|
1
|
225
|
June 12, 2023
|
|
Erro no Cluster.split
|
|
1
|
148
|
May 2, 2023
|
|
The cluster step failed
|
|
1
|
177
|
May 2, 2023
|
|
Query for extended hours in cluster.seqs command for 16S RNA sequence reads
|
|
1
|
194
|
May 2, 2023
|
|
Permanova for Beta diversity
|
|
4
|
916
|
May 2, 2023
|
|
Stability.trim.contigs.fasta is blank, aborting
|
|
3
|
229
|
April 24, 2023
|
|
Adapter, Primer removal
|
|
6
|
365
|
April 18, 2023
|
|
Size of reference library (linear or non-linear?)
|
|
3
|
200
|
April 18, 2023
|
|
How to determine start and end point for screen.seq command
|
|
4
|
213
|
April 11, 2023
|
|
So many sequences removed with maxambig=0
|
|
5
|
324
|
March 21, 2023
|
|
Query regarding Taxonomy file generation in mothur
|
|
1
|
207
|
March 9, 2023
|