AVITI vs. MiSeq

Hi everyone,

Our Genomics Center has been advertising using Element Biosciences AVITI system for 16S sequencing. Apparently, it’s cheaper and produce higher quality reads. I’m just checking to see if anyone has analyzing sequencing data from AVITI using Mothur. Any differences? Things to watch out for?
Thanks much.

Hi there,

I’d never heard of it before your posting (thanks!). It looks a lot like Illumina. I would want to see them sequence a mock community to get the actual error rates. If they’re doing standard paired end sequencing along the lines of what we do in Kozich then mothur should be able to handle it. But if it’s their LoopSeq approach, then that might require doing some assembly elsewhere before coming into mothur.

Pat