Uchime/vsearch executables do not "exist"

Hi all,

I’m getting the following error(s):

uchime file does not exist. Checking path…
[ERROR]: file does not exist. mothur requires the uchime executable.
[ERROR]: did not complete chimera.uchime.

Same for vsearch.

I’m baffled, because the mothur executable and the uchime/vsearch binaries are all in the same folder. This happens with mothur 1.39.5 and 1.42.3.

Have you tried using the vsearch parameter option in the chimera.vsearch command?


The vsearch parameter allows you to specify the name and location of your vsearch executable. By default mothur will look in your path and mothur’s executable location. You can set the vsearch location as follows: vsearch=/usr/bin/vsearch.

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