Hey, i’m trying to denoise my sequence data but am having troubles. I’m using the mothur 1.24.1. When I trim.flows with 1 sample things are fine. When i include more than 1 sample, trim.flows doesn’t finish and fills up my harddrive with the scrap file (left it for a couple of hours yesterday and the scrap file was 30gb)
one file
mothur > trim.flows(flow=454Reads.ABI1.flow, oligos=abi1.txt, maxhomop=8, pdiffs=2, maxflows=450)
5257
Output File Names:
E:\mothur124\commeco\454Reads.ABI1.trim.flow
E:\mothur124\commeco\454Reads.ABI1.scrap.flow
E:\mothur124\commeco\454Reads.ABI1.ABi1.flow
E:\mothur124\commeco\454Reads.ABI1.flow.files
all files (30 samples)
mothur > trim.flows(flow=commeco.flow, oligos=commeco.oligo, maxhomop=8, pdiffs=3, maxflows=450)
10000
…
1070000… I killed mothur at this stage (scrap file was at 4gb)