Hi, mothur team
I’m using the latest version of mothur and I have tried trimming of my sequences, all the sequences get scrapped.
The command is:
mothur > trim.flows(flow=0460_min200_c.flow, oligos=0460.oligos, pdiffs=2, bdiffs=1, processors=2)
2553
Output File Names:
0460_min200_c.trim.flow
0460_min200_c.scrap.flow
0460_min200_c.flow.files
mothur > shhh.flows(file=0460_min200_c.flow.files, processors=2) [ERROR]: 0460_min200_c.flow.files is blank. Please correct.
my oligos file like this:
forward GGACTACVSGGGTATCTAAT
#reverse GTGCCAGCMGCCGCGGTAA
barcode ACACGT 4000
barcode ACGTAC 4001
barcode ACTGCA 4002
barcode AGAGTC 4003
barcode AGCTGA 4004
barcode AGTCAG 4005
barcode ATATCG 4006
barcode ATCGAT 4007
barcode ATGCTA 4008
barcode CACAGT 4009
barcode CAGTCA 4010
barcode CATGAC 4011
barcode CGATAT 4012
barcode CGCGCG 4013
barcode CGTATA 4014
barcode GACTAG 4015
barcode GAGATC 4016
barcode GATCGA 4017
barcode GTACAC 4018
barcode GTCACA 4019
barcode GTGTGT 4020
barcode TACGTA 4021
barcode TAGCAT 4022
barcode TATACG 4023
Any ideas what the problem may be?
Thanks! 