Blank output after applying trim.flow

mothur bugs
1

Hello,

I have a problem with the command trim.flow. I just followed the steps in 454 SOP to trim my data and the mothur scraped all the flows after running the command:
trim.flows(flow=66Samples_7.flow,oligos=66Samples_7.oligos,pdiffs=2,bdiffs=1,processors=8)

I have tried to use the order=B, but still doesn’t work. :oops:

2

Could you post the first few lines of your flow file as well as your oligos file? What version of mothur are you using?