I am pretty new to mothur. I have some older 454 data sets that need denoising. I created my oligos file as described on the website, but the trim.flows creates an empty all.flow.files file? Has anyone else encountered a similar problem? the oligos file was in .txt format- I saw some tutorials online using it- could that be a problem? Otherwise how can I change this to an oligos format- if that is the problem?
my oligos file looks something like this:
barcode ACTGCAGT P3.1.B
barcode ACTGCTCT P3.2.B
barcode ACTGCTGA P3.3.B
Thank you so much, Mel