mothur

Summary.seqs file mismatch detected

Hello,

I am removing chimeras and singletons via chimera.vsearch and split.abund in mothur. After split.abund, I used summary.seqs, but it gave me an error message:
[ERROR}: Your name file contains 175504 sequences, but your fasta file contains 173083. File mismatch detected, quitting command.

I tried this multiple times and also made sure the commands were completely finished. I also used only 1 processor.

Please help!

Thanks,
Kus

Can you post the exact syntax of the commands you are running? (Also, removing singletons will skew the shape of the communities and impose a bias against those samples with fewer sequences).

Pat