I´m exactly following MiSeq SOP to analyze my data. I firstly analyzed a batch of 4 samples and everything worked well. I´m now trying to analyze a batch of 22 samples and…
When running summary.seqs after align.seqs I find the following error:
mothur > summary.seqs(fasta=stability.trim.contigs.good.unique.align, count=stability.trim.contigs.good.count_table)
Using 24 processors.
[ERROR]: ‘M06398_30_000000000-J88C7_1_1104_21092_24568’ is not in your name or count file, please correct.
[ERROR]: Your name file contains 157162 unique sequences, but your fasta file contains 1. File mismatch detected, quitting command.
I checked
summary.seqs(fasta=stability.trim.contigs.good.unique.align)
and the result is that the file contains 310927 sequences
as well as
summary.seqs(fasta=stability.trim.contigs.good.unique.fasta, count=stability.trim.contigs.good.count_table)
and the result is that the file contains 310927 unique sequences of a total of 955818 sequences.
and
summary.seqs(fasta=stability.trim.contigs.good.unique.align, name=stability.trim.contigs.good.names)
and the result is that the file contains 310927 unique sequences of a total of 955818 sequences.
Thus, I can not find where is the problem.
I would appreciate any help.