mothur

Make.sra - 'missing project name'

Hi! I’m trying to run make.sra to format my files for NCBI submission. I’ve used this command previously without any issues. However, this time I keep getting the following error:

mothur > make.sra(file=antibiotics1.files, project=antibiotics1.project, mimark=antibiotics1.tsv)
[ERROR]: missing project name from project file, quitting.
[ERROR]: missing required fields for package, please correct. Required fields are sample_name, description, sample_title, collection_date, env_biome, env_feature, env_material, geo_loc_name
, lat_lon, seq_methods, organism. Missing sample_name, description, sample_title, collection_date, env_biome, env_feature, env_material, geo_loc_name, lat_lon, seq_methods, organism

I used get.mimarks() to make my mimark file and I have double checked that both files are completely filled out and are tab delimited. I’m not sure why mothur isn’t recognizing the fields. I have tried this command on both version 1.39.5 and 1.40.5.

Thank you for the help!

Kelsy

Can you post the contents of antibiotics1.project?

Thanks,
Pat