I’ve created the project and mimarks files for make.sra using mothur’s instructions. My samples are named with a {letterNumber} structure; e.g., A001, A002, … When I run make.sra, I get the following message: “A002 is a duplicate sample to A001. It has all the same attributes in the MIMarks file. Samples must have distinguishing features to be uploaded to the NCBI library, please correct.”
Thanks for sending your files. The issue is caused by the mimarks file entries. NCBI requires samples to contain different information in the mimarks file. Looking at the samples in question:
ARM001
ArtPrize study samples
missing
MiSeq V4
human skin metagenome
2015
human-associated habitat
organic feature
organic material
United States of America: Michigan
Homo sapiens
42.9607 N 85.4955 W
forearm
ARM002
ArtPrize study samples
missing
MiSeq V4
human skin metagenome
2015
human-associated habitat
organic feature
organic material
United States of America: Michigan
Homo sapiens
42.9607 N 85.4955 W
forearm
The samples are identical in the mimarks file. You need to modify the mimarks file to distinguish between the samples.