I’m new with submitting Illumina (MiSeq) sequences to the NCBI. A colleague suggested me to try the command make.sra as it is super easy to use.
However, I’m struggling with my mimark file at the moment… I filled the template as described on the Wiki page… but I get an error message I run the make.sra command :
[ERROR]: MIMarks file is missing group 16S_BGR_B1, please correct.
[ERROR]: MIMarks file is missing group 16S_BGR_B2, please correct.
[ERROR]: MIMarks file is missing group 16S_BGR_B3, please correct.
[ERROR]: MIMarks file is missing group 16S_BGR_S1, please correct.
… (for all the samples) - as you can guess, 16S_BGR… are the sample ID (were the same in the oligos file, and in the fastq containing file).
I don’t understand which information is missing in the mimark file because I filled all the required (*) fields for each sample…