I have just started using mothur and enjoyed it so far.
The analysis example are of great help.
With my data, i am haveing the following issues:
- Tried to run the make.share command,
make.shared(list=002.an.list, group=002.groups, label=0.03)
but it gives me the error : [ERROR]: Could not open 002.an.27F/534R_001.rabund
for all individual samples, but creates output files: 002.an.27F/534R_001.rabund for all individual samples whihc i dont see in my directory. there is the common 002.an.shared file too.
- when i try this : > collect.single(shared=002.an.shared, calc=chao-invsimpson, freq=5)
gives me an error
[ERROR]: Could not open 002.an.27F/534R_001.rabund
i compared all my file with the example file and they are ok.
Please let me know if i am missing something here. my dist.seqs and cluster commands did not had any issues.