make.shared

Commands in mothur
1

Hi mothur group

I am following 454SOP to analysis my data.
When i go

mothur > cluster(column=ECSto.unique.filter.dist, name=ECSto.names)
mothur > dist.seqs(fasta=ECSto.unique.filter.fasta, cutoff=0.03, processors=3)
mothur > make.shared(list=ECSto.unique.filter.an.list, group=ECSto.groups, label=0.03)

i got following error

[ERROR]: JOSNX1G02JZXK8 is in your groupfile and not your listfile. Please correct.
[ERROR]: JOSNX1G02JZXNB is in your groupfile and not your listfile. Please correct.
[ERROR]: JOSNX1G02JZY90 is in your groupfile and not your listfile. Please correct.
[ERROR]: JOSNX1G02JZYPD is in your groupfile and not your listfile. Please correct.
[ERROR]: JOSNX1G02JZYXM is in your groupfile and not your listfile. Please correct.
[ERROR]: JOSNX1G02JZZQN is in your groupfile and not your listfile. Please correct.
Your group file contains 371555 sequences and list file contains 37540 sequences. Please correct.

Output File Names:
ECSto.unique.filter.an.shared
ECSto.unique.filter.an.MID01.hap18SF.rabund
ECSto.unique.filter.an.MID02.hap18SF.rabund
ECSto.unique.filter.an.MID03.hap18SF.rabund
ECSto.unique.filter.an.MID04.hap18SF.rabund

That means i should make group file and list file both have the same sequence number
but what step should i do…?

2

I got answer

run
unique.seqs(fasta=sample.fasta, name=sample.names)
list.seqs(name=sample.names)
get.seqs(accnos=sample.accnos, group=sample.groups)

make.shared(list=sample.an.list, group=sample.pick.groups, label=0.03)