Hello everyone,
I am using the following command:
get.sharedseqs(group=E:\Phylotype_Bitburger\Phylotype_V1V3\ISOX32Y01.shhh.good.pick.groups, list=E:\Phylotype_Bitburger\Phylotype_V1V3\ISOX32Y01.shhh.trim.unique.good.filter.unique.precluster.pick.pds.wang.tx.list, label=1, sharedgroups=LIMS_2108739-LIMS_2108742-LIMS_2108745)
If i understand the command right i only get sequences shared by the three groups. When looking at the output file i see the following:
ISOX32Y01C1125|LIMS_2108739|Otu003
ISOX32Y01C2L1L|LIMS_2108742|Otu003
ISOX32Y01C2S7I|LIMS_2108739|Otu003
ISOX32Y01C39SF|LIMS_2108742|Otu003
ISOX32Y01C4A9K|LIMS_2108745|Otu003
ISOX32Y01C4M8C|LIMS_2108740|Otu003
ISOX32Y01C4O6V|LIMS_2108742|Otu003
The group LIMS_2108740 is present but should be excluded. What´s wrong with me?
Thanks in advance
Andre