Hi, I am processing a Miseq on my Mac.
After running filter.seqs with aligned sequences, I got the results like below.
mothur > filter.seqs(fasta=all_H.trim.contigs.good.unique.good.align, vertical=T, trump=.)
Length of filtered alignment: 610
Number of columns removed: 12814
Length of the original alignment: 13424
Number of sequences used to construct filter: 3030962
Output File Names:
Afterwards, I did run unique.seqs with filtered fasta file and it seemed go through the process. However, the process freezes at some point and I can’t go further. Mac was fine though.
mothur > unique.seqs(fasta=all_H.trim.contigs.good.unique.good.filter.fasta, count=all_H.trim.contigs.good.good.count_table)
[stopp and freezes at this point]
Anyone could tell me what is wrong on this??
I would appreciate if you could help me.