mothur

The command unique.seqs(fasta=stability.trim.contigs.good.fasta) is suddenly killed

unique.seqs(fasta=stability.trim.contigs.good.fasta)
when I executed this command, the command is suddenly killed. why? Really confused and stuck. Please help. Thank you!

Hey there - I suspect it’s because you have so many uniques for the amount of RAM on your computer. What type of data are these and from what type of environment?

Thank you. I believe that you are right. but how should I deal with it ?

I’d classify everything and then assign sequences to phylotypes using the phylotype function. I talk about all this here: Why do I have such a large distance matrix

Pat

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