Unable to open files

mothur bugs
1

Hi, I am learning to handle mother in Mac. My problem is that even I operate according to 454 SOP in the website, and make sure the files having put under the same file box, I cannot open all the files. Below is one of the examples.

mothur > trim.seqs(fasta=GQY1XT001.fasta, oligos=GQY1XT001.oligos, maxambig=0, maxhomop=8, flip=T, bdiffs=1, pdiffs=2, keepfirst=200, processors=2)

Unable to open GQY1XT001.fasta
Unable to open GQY1XT001.oligos

Using 2 processors.
[ERROR]: did not complete trim.seqs.

Hope somebody give me advices urgently!

Thank you so much!

2

Within mothur can you type the following?

system(ls)

Do you see GQY1XT001.fasta and GQY1XT001.oligos? If not, you’re in the wrong folder and need to navigate to wherever they are and run mothur.

Pat

3

Thank you very much!
I can do it after try your advice!!