_Hi there,
I am starting as a user for MiSeq data with mothur-therfore I am following the MiSeq SOP
I have downloaded the Example data from Schloss lab “MiSeq_SOP” into my home “/Users/kimlema/”.
I ran the following:_
mothur > make.contigs(file=stability.files)
Unable to open stability.files. Trying default /Users/kimlema/mothur/stability.files
Unable to open /Users/kimlema/mothur/stability.files
Using 1 processors.
[ERROR]: did not complete make.contigs.
So then I ran this one:
mothur > make.contigs(file=MiSeq_SOP/stability.files)
and got:
Using 1 processors.
Unable to open F3D0_S188_L001_R1_001.fastq. Trying default /Users/kimlema/mothur/F3D0_S188_L001_R1_001.fastq
[WARNING]: can’t find /Users/kimlema/mothur/F3D0_S188_L001_R1_001.fastq, ignoring pair.
Unable to open Mock_S280_L001_R2_001.fastq. Trying default /Users/kimlema/mothur/Mock_S280_L001_R2_001.fastq
[WARNING]: can’t find /Users/kimlema/mothur/Mock_S280_L001_R2_001.fastq, ignoring pair.
It took 0 secs to process 0 sequences.
Output File Names: MiSeq_SOP/stability.trim.contigs.fasta MiSeq_SOP/stability.trim.contigs.qual MiSeq_SOP/stability.contigs.report MiSeq_SOP/stability.scrap.contigs.fasta MiSeq_SOP/stability.scrap.contigs.qual
So how does mothur searches for the files?
Thanks!!