trim.flows unable to open my flow file and oligo file

Commands in mothur
1

Hi,
I am trying to open the Lam_bacteria_SFF.flow file generated using sffinfo command,but it is not accepting the file,could you please help me to execute my files.
Thanks,
srisailam.
mothur > trim.flows(flow=Lam_bacteria_SFF.flow, oligos= Lam_bacteria1.oligos,minflows=200, maxflows=500, pdiffs=2, bdiffs=1, processors=2)

Unable to open Lam_bacteria_SFF.flow
Unable to open Lam_bacteria1.oligos

Using 2 processors.
[ERROR]: did not complete trim.flows.

mothur > trim.flows(flow=Lam_bacteria_SFF.flow, oligos= Lam_bacteria1.oligos,minflows=200, maxflows=500, pdiffs=2, bdiffs=1, processors=4)

Unable to open Lam_bacteria_SFF.flow
Unable to open Lam_bacteria1.oligos

Using 4 processors.
[ERROR]: did not complete trim.flows.

mothur > trim.flows(flow=Lam_bacteria_SFF.flow, oligos= Lam_bacteria1.oligos,minflows=200, maxflows=500, pdiffs=2, bdiffs=1, processors=4)

Unable to open Lam_bacteria_SFF.flow
Unable to open Lam_bacteria1.oligos

Using 4 processors.
[ERROR]: did not complete trim.flows.

2

You’re likely in the wrong folder. From within mothur can you type:

system(ls) [for mac/linux]

or

system(dir) [for windows]

3

Hi,
Thank you of your help.when I typed the command it shows like this.
regards,
srisailam.
mothur > system(ls)

Lam_bact_codes.oligos
Lam_bacteria.oligos.txt
Lam_bacteria1.oligos.txt
Lam_bacteria_SFF.fasta
Lam_bacteria_SFF.flow
Lam_bacteria_SFF.qual
Lam_bacteria_SFF.sff
Lam_bacteria_SFF.summary
Lam_oligos.txt
commandScreen.output
mothur.1407945143.logfile

4

Looks like you might have skipped the sffinfo step