Hello ,
I am getting this error while running classify command
mothur > classify.seqs(fasta=unique_ASVs.fasta, reference=/home/adyasha/18S/data/silva.seed_v138_2.align, taxonomy=/home/adyasha/18S/data/silva.seed_v138_2.tax, cutoff=60, probs=FALSE, processors=4)
[ERROR]: has occurred in the MothurOut class function mothurConvert. Please contact Pat Schloss at mothur.bugs@gmail.com, and be sure to include the mothur.logFile with your inquiry.
Please help me on this.
Thank you.
The error message indicates mothur likely had trouble reading the taxonomy file. This can happen if part of the taxonomy string is mistaken for a confidence score. Did you generate the reference and taxonomy files yourself?
Hi,
Thank you for the reply. I have downloaded the mothur supported silva database files. I have used this link
I have downloaded it and gave it as inputs for the above mentioned command
I am able to run classify.seqs without error using those reference files. Are you using our most current release?
I’m using the one mentioned in the above link.
It’s 138.2 I guess.
Please share the latest one that’s mothur supported.
I will try using that .
Thank you.
Here’s a link to the latest version of mothur, 1.48.2.
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