Hi everyone,
i’m starting during these days to get into mothur. Reading the mothur tutorial for Ion Torrent data at the first trim step (trim.flows) thay suggest to use “order=I” for the Ion Torrent data. If i understood this command it is the nucleotide flow order doesnt it? ok so ion torrent have different flows order for their chip (316 or 318) so which is the order that the command “order=I” take into account?
Thank you