Ion Torrent


My lab recently received Ion torrent data that we are going to analyze using Mothur. We received fastq files from them with the barcodes removed but not the primers. I am unfamiliar with Ion torrent and have only done 454 anaylsis in the past. I used the command to generate fasta files and qual files and did a summary seqs and my samples seem to have a good number of sequences per sample. I just do not know where to go next. I do not have a .flow file (nor do I know how to make one) in order to continue with the ion torrent protocol on the wiki.

I would suggest going back to the sequencing vendor and asking for the original sff file. IonTorrent files are analogous to 454 files. They really should provide you with the original data.