I have processed my 16S V4 paired-end sequence data using the mothur SOP in version 1.47.0 and now am on the analysis stage. I want to run my mothur output biom and fasta files through PICRUST2 to take a look at the predicted functional abundances. However, I have found that the fasta file contains sequence IDs that look like this: >MISEQ_159_000000000-JJ3F9_1_1106_9289_25841 and the biom file only contains the OTU IDs (Otu00001) so there is an ID mismatch between these files and therefore it won’t run in PICRUST2. Do you know how I can get the IDs to match up?
Thanks in advance.