mothur

Biom file for picrust2

Hello Pat and mothur community, I ran into a file mismatch error in picrust2 which I don’t know how to fix. The issue comes from the fact that in the ASV.fna input file (generated in mothur from the screened/filtered/preclustered fasta file using consensus.seqs and label=0.03) the name of the consensus sequences are mothur OTU numbers, such as Otu00001 etc. In contrast, in the .biom file generated with make.biom the OTUs have greenegenes IDs. Not surprisingly, picrust2 program metagenome_pipeline.py throws an error (ValueError: No sequence ids overlap between the input files). Can’t figure out how to generate the ASV.fna file with greengenes sequence IDs.
many thanks,
Giovanni

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