I’m not sure this is the best place to ask this, but I figured it was worth a shot.
I generated a .biom table using the .cons.taxonomy I generated in mothur. I used the GreenGenes version specified by Galaxy (13.5) and I removed the unknown taxa using the remove.lineages command during this process. I input this file into the Galaxy online version to run PICRUSt. It generates this error when I try to use normalize by copy number:
ValueError: No OTUs match identifiers in precalculated file. PICRUSt requires an OTU table reference/closed picked against GreenGenes.
Example of the first 5 OTU ids from your table: Otu02710, Otu02711, Otu02712, Otu02713, Otu02714
I don’t understand why this error comes up or why it can get through 2709 OTUs before stopping. Any thoughts/ideas/suggestions would be super helpful and much appreciated! I tried getting help from the lab that runs Galaxy but haven’t received a response.