align.seqs error

I get the following error when running align.seqs:
[ERROR]: Could not open Gary.trim.unique.align316.num.temp
[ERROR]: Could not open Gary.trim.unique.align316.num.temp

Any suggestions?

It looks like your are running align.seqs with multiple processors and one of the processes encountered an error and failed. If you send your logfile and input files to I can try to track down the problem for you.

I’m encountering a similar problem with the Green geens reference file.

mothur > align.seqs(fasta=P004.trim.contigs.good.unique.fasta, reference=gg_13_5_99.fasta)

Using 1 processors.

Reading in the gg_13_5_99.fasta template sequences… [ERROR]: template is not aligned, aborting.

I’ve also triedgg_13_8_99.fasta as well

We haven’t posted the aligned version. The version we posted really is only appropriate for classifcation.