mothur

Unable to perform Make.contigs

Commands in mothur
#1

Dear all,
I am trying to create contig from forward and reverse files. But Mothur app is closing. I am unable to perform.

mothur > make.contigs(ffastq=/Users/apple/Dropbox/KF_S_S4_L001_R1_001.fastq, rfastq=/Users/apple/Dropbox/KF_S_S4_L001_R2_001.fastq)

dyld: lazy symbol binding failed: Symbol not found: ____chkstk_darwin

Referenced from: /Users/apple/Downloads/mothur1.44/mothur

Expected in: /usr/lib/libSystem.B.dylib

dyld: Symbol not found: ____chkstk_darwin

Referenced from: /Users/apple/Downloads/mothur1.44/mothur

Expected in: /usr/lib/libSystem.B.dylib

Abort trap: 6

logout

Saving session…

…copying shared history…

…saving history…truncating history files…

…completed.


#2

What version of mothur are you running and what version of OS X ? Mothur requires at least OS X 10.14

#3

Dear Westcott,
I am using Mac OS 10.12 Sierra with Mothur 1.44.
Regards,
Dinesh

#4

Dear Westcott,
I am getting error with Mothur 1.39.5 also

mothur > make.contigs(ffastq=/Users/apple/Dropbox/arctic/KF_S_S4_L001_R1_001.fstq, rfastq=/Users/apple/Dropbox/arctic/KF_S_S4_L001_R2_001.fastq, processors=4)

Using 4 processors.

M01522_253_000000000-C44KY_1_1101_9507_1894 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.

M01522_253_000000000-C44KY_1_1119_8917_7187 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.

M01522_253_000000000-C44KY_1_2109_15693_21858 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.

Making contigs…

Segmentation fault: 11

logout

Saving session…

…copying shared history…

…saving history…truncating history files…

…completed.

Deleting expired sessions…none found.

#5

The version of OS X is the issue. Mothur is built for 10.14 or greater. I recommend upgrading your version of OS X. https://www.apple.com/macos/how-to-upgrade/