Hi all,
I have to admit I am struggling through mothur, the tutorial was a breeze. I am sure there will be light at the end of the tunnel, and hopefully someone can give a helping hand.
Background information: We have miseq 16S data with beautiful R1 reads and horrible R2 reads. We dumped the R2 reads and the R1 read (fasta and qual) was reverse complemented to provide the R2 reads for Mothur.
Everything appears to have gone dandy until we hit summary.seqs after count.seqs as shown below. We have 94 incidences of
[ERROR]: is not in your name or count file, please correct.
I would be so grateful if someone could provide a solution on how to correct this.
Much appreciated,
C.
PS: Excerpt from Mothur logfile
count.seqs(name=kalydeco.trim.contigs.good.names, group=kalydeco.contigs.good.groups, processors=47)
Using 47 processors.
It took 137 secs to create a table for 7659463 sequences.
Total number of sequences: 7659463
Output File Names:
kalydeco.trim.contigs.good.count_table
mothur > summary.seqs(count=kalydeco.trim.contigs.good.count_table, fasta=kalydeco.trim.contigs.good.fasta)
Using 11 processors.
[ERROR]: ‘M01503_88_000000000-A97TN_1_1112_19147_20815’ is not in your name or count file, please correct.
Using 11 processors.
[ERROR]: ‘M01503_88_000000000-A97TN_1_2112_13774_20031’ is not in your name or count file, please correct.
Using 11 processors.
[ERROR]: ‘M01508_32_000000000-A97R4_1_1106_10761_5448’ is not in your name or count file, please correct. . . . . . . .
. . . . . . . . . this continues for a total of 94 sequence IDs