Hi,
I’ve run the chimera.uchime and then the remove.seqs commends. I’d now like to split my dataset back into groups.
I have tried the split.groups command but I’m getting the error
Error in reading your fastafile, at position -1. Blank name.
I’m not quite sure what I have done wrong.
Any suggestions?
Thanks
Beth
Here is a more detailed account of the error…
mothur > split.groups(fasta=stability.trim.contigs.good.unique.pick.fasta, count=stability.trim.contigs.good.count_table)
[ERROR]: Could not open stability.trim.contigs.good.unique.pick.aR1-5.fasta
.
.
. (same thing for all groups reported)…
[ERROR]: Could not open stability.trim.contigs.good.aWT12-6.count_table
[ERROR]: Could not open stability.trim.contigs.good.unique.pick.aWT12-7.fasta
[ERROR]: Could not open stability.trim.contigs.good.aWT12-7.count_table
[ERROR]: Could not open stability.trim.contigs.good.unique.pick.fasta
Error in reading your fastafile, at position -1. Blank name.
When I watch the folder it is working in, it briefly makes all these files and then deletes them.
I had done the same thing on a test set that worked fine. I am not sure why it is not working now.
From the log file…
Mac version
Using ReadLine
Running 64Bit Version
mothur v.1.32.1
Last updated: 10/16/2013
Can you try running the command with our current version, 1.38.1 https://github.com/mothur/mothur/releases?