I’m a new mothur user using the version 1.43.0 and I’m not sure it’s a bug but I always get this error message after phylogenetic command:
Blockquote mothur > dist.seqs(fasta=stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.pick.fasta, output=lt, processors=8)
[ERROR]: stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.pick.fasta is blank. Please correct.
Using 8 processors.
Sequence Time Num_Dists_Below_Cutoff
I followed the steps on MiSeq_SOP wifi page and the same error message appears when I try it with example data from Schloss lab. And if I just skip phylogenetic steps and use analysis commands it says it’s unable to open stability.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.pick.opti_mcc.shared.
I would like to upload a logfile so it might be easy to troubleshoot but I don’t know how to upload since it doesn’t allow me to upload other than jpg file.
Any idea why this happens? Thanks!