From the MiSeq SOP I’ve been convinced that it is important to include a Moch standard in the setup. However I don’t know how to proceed to construct the fasta-file I need for calculating sequencing errors etc. The Mock community we were able to get hold on is called ZymoBIOMICS™ Microbial Community Standard. Could anyone help me out, please!
they give a link in the manual
https://s3.amazonaws.com/zymo-files/BioPool/ZymoBIOMICS.STD.genomes.ZR160406.zip
Thanks a lot! That was very helpful!! 