Hello! I have used the ZymoBIOMICS Microbial Community Standard II as my mock community for a recent MiSeq run. I am stuck at seq.error
The command requires a reference FASTA and Zymo provides individual 16S&18S rRNA sequences (FASTA format) and genomes (FASTA format) for each strain. Do I need to combine those files to make one reference file or can I somehow include multiple references?
This is the provided instruction manual for that particular community: https://files.zymoresearch.com/protocols/d6310_zymobiomics_microbial_community_standard_ii(log_distribution).pdf