So I used the ZymoBIOMICS Microbial Community DNA Standard as a mock community and downloaded their fasta files as a zip file, but I’m not sure how to create a full reference fasta file from that for use in the seq.error command. I attended your workshop about a year and a half ago and I know you used a file labeled like HMP… and when I opened that up it seemed to contain the fastas for all the bacteria. So do I need to somehow combine all the fastas I downloaded from Zymo into one fasta file? Is they any other type of formatting I need to do?
Hi there - yeah, you’ll need to merge all of the sequence data into a single file that looks like the old HMP fasta file.
Pat